肥胖小鼠 z-bo 2020-11-25 310

　　Objective 　 Apply hypoxia training to obese mice to observe the changes in the levels of Ghrelin and hypothalamic GHSR in gastric tissue to explore whether hypoxia training affects Ghrelin￣ GHSR pathway to improve obesity.

　　Methods Male C57BL/6J mice were randomly divided into normal control group (C, n=8) and high-fat diet group (H, n=52). After the obesity model was established in H group, they were randomly divided into obesity control group (HC) , Obesity normoxic exercise group (HE), obesity hypoxic exposure group (HH) and obesity hypoxic exercise group (HHE), each exercise group is subjected to moderate-intensity treadmill training, and each hypoxic group is subjected to intermittent hypoxic exposure (11. 2% oxygen content), record weekly weight and food intake for 4 weeks, test serum TC, TG, GLU and total Ghrelin levels, RT-PCR method to detect hypothalamic GHSR and gastric Ghrelin mRNA expression levels, WB detects hypothalamic GHSR and GHSR NPY protein content and gastric tissue Ghrelin, Goat and HIF-2α protein content.

　　Results (1) After 4 weeks of intervention, the weight of the HE, HH, and HHE groups was significantly lower than that of the HC group. In the initial stage of hypoxia intervention, the food intake of the HH and HHE groups decreased, but with the extension of the intervention time, the obese groups The food intake of the HC group is gradually approaching. (2) The blood indexes of the HC group are significantly higher than those of the C group. The TC level of the HH group is significantly lower than that of the HC group. The GLU level of the HE, HH, and HHE groups is significantly lower than that of the HC group. The total Ghrelin level was significantly lower than that in the HC group. (3) The levels of hypothalamic GHSRMRNA and gastric GhrelinRNA in the HC group were significantly lower than those in the C group. The levels of hypothalamic GHSRMRNA and the HHE group in the HH and HHE groups were significantly higher than those in the HC group. Hypothalamic GHSR protein levels in HE, HH, and HHE groups were significantly higher than those in HC group, and NPY protein levels in HE and HHE groups were significantly higher than those in HC group. (5) The gastric Ghrelin protein levels in HE, HH, and HHE groups were significantly higher than those in HC group. The Goat protein level in the HE group and the HHE group was significantly higher than that in the HC group, and the HIF-2α protein level in the HH group and the HHE group was significantly higher than that in the HC group.

　　Conclusion　 Hypoxic training can affect the metabolism of glucose and lipids by regulating the Ghrelin￣GHSR signaling pathway, thereby reducing the weight of obese mice.