异丙肾上腺素,特异性心肌缺血 z-bo 2020-11-27 382

　　Objective To observe the expression of HE staining, VEGF (vascular endothelial growth factor) and FCF (fibroblast growth factor) in myocardial tissue slices caused by subcutaneous injection of isoproterenol for different days (1 mg/kg body weight) induced specific cardiomyopathy in rats And changes in the T wave of the ECG.

　　Methods Except for the blank group, rats in each group were injected subcutaneously with multiple points of isoproterenol (1mg/kg), respectively for the 2d group of continuous subcutaneous multiple points of isoproterenol (1mg/kg), and continuous subcutaneous multiple points of isoproterenol injection In the 4d group of steroid (1mg/kg), continuous subcutaneous injection of isoproterenol (1mg/kg) for 6d group. One week after the administration, the rats were anesthetized, the electrocardiogram was measured, and the heart was taken. HE staining was used to observe the changes in the degree of injury of rat myocardial cells, and immunohistochemical staining was used to observe the myocardial tissue sections VEGF (vascular endothelial growth factor) and FGF Fibroblast growth factor) expression changes.

　　Results Compared with the blank control group, the HE-stained tissue slices in the 6-day continuous subcutaneous injection of isoproterenol (1 mg/kg) were significantly different from those in the blank control group. At the same time, the model group’s myocardial tissue slices VEGF (vascular There are also significant differences in the expression of endothelial growth factor and FCF (fibroblast growth factor) compared with the blank control group.

　　Conclusion The results of this study show that compared with the blank control group, continuous subcutaneous injection of isoproterenol (1mg/kg) for 6 days in the model rats caused by specific cardiomyopathy in myocardial tissue sections HE staining and VECF (vascular endothelial growth factor) There are significant differences in the expression of FGF (fibroblast growth factor) and the changes in the T wave of ECG. Therefore, continuous subcutaneous injection of isoproterenol (1 mg/kg) for 6 days in rats can be used as an experiment for evaluating the efficacy of specific cardiomyopathy. Use of animal models.