动物模型,大鼠脑瘫 z-bo 2020-07-29 649

　　1. Experimental cerebral palsy animal model after asphyxia in newborn rats:

　　Under awakening local anesthesia, the newborn rat is ligated right common carotid artery, and then placed in a constant temperature airtight glass container with 8% oxygen +. Two hours after the location, the 92% nitrogen mixture was taken out to observe the behavior of the young mice, and then the brains of each group were decapitated.

　　2. Rat central spastic paralysis model: The above-mentioned upper limb spastic cerebral palsy rats were randomly divided into two groups according to the order of operation, each with 8 rats. Experimental group: C6 nerve roots were cut off.

　　3. Improve rat spastic cerebral palsy model:

　　Wistar rats, male and female, 200-250g, pentobarbital 30mg/kg intraperitoneal anesthesia. Make a vertical incision on the sagittal suture above the skull to expose the anterior anterior... The modified degumming positioning system is used to select 6.6 mm behind the front fork and 1 mm to the left of the sagittal suture. Use a small electric drill to make a hole about 2 mm in diameter in the skull plate. , Insert an anode needle electrode with a diameter of about 0.5 mm into 9.7 mm vertically, and connect the cathode to the tail of the mouse. Apply direct current to destroy the vertebral body. After stimulation, the electrode was pulled out, the incision was closed, the rat was killed immediately and the specimen was collected.