大鼠肺动脉高压模型 z-bo 2020-12-01 355

　　Objective: To investigate the expression level and role of RhoA/ROCK signaling pathway in a rat pulmonary hypertension model related to left heart disease.

　　 Method: 20 male SD rats aged 3~4 weeks, weighing 90~100 g, were randomly divided into control group (group C: n = 10) and pulmonary hypertension group (group H: n = 10). In group H, a rat model of left heart disease-related pulmonary hypertension was constructed by the fixation and constriction of the ascending aorta. The rats in group C were treated with sham operation (titanium clamps were fixed to the paravascular mediastinum tissue instead of the ascending aorta. All other operations were the same as those in H Group), 60 days after modeling, the rats in each group were tested for hemodynamics (right ventricular systolic pressure, pulmonary artery pressure), and the rats were sacrificed and in vivo cardiopulmonary lavage with PBS caused both lungs to become white, and the left lung Tissues were fixed in 4% paraformaldehyde for pathological sections to observe the pathological changes of lung tissues, and the right lung tissues were frozen for biomolecular detection (Rho kinase mRNA, RhoA mRNA, ET-A receptor mRNA). Results: Compared with group C, pulmonary artery pressure and right ventricular systolic pressure in group H were significantly increased (P<0.01), pulmonary arteriole walls were significantly thickened, pulmonary arterioles were narrowed or even occluded, and wall hypertrophy index was significantly increased ( P<0.01); Compared with group C, the expression level of Rho kinase mRNA in the lung tissue of group H increased significantly, and the expression levels of RhoA mRNA and ET-A receptor mRNA also increased significantly, and the differences were statistically significant (P<0.01) . Conclusion: A rat model of left heart disease-related pulmonary hypertension was successfully constructed by using ascending aorta fixation and constriction; compared with group C, the walls of small pulmonary blood vessels in group H were significantly thickened, and Rho kinase mRNA, RhoA mRNA, ET in lung tissue -A receptor mRNA expression is significantly increased, this signaling pathway may be involved in the formation of left heart disease-related pulmonary hypertension.