骨肉瘤模型 z-bo 2020-12-01 337

　　Objective: To explore the key factors for the success rate of tumor-bearing animal models of osteosarcoma.

　　 Method: 24 C3h mice aged 4 to 8 weeks, including 6 C3h/HeJ type and 18 C3h/HeN type, were randomly divided into 2 groups. Group A was injected with a general depilatory cream and injected 0.2 mL of LM8 cell suspension at a concentration of 5×107 orders/mL into the lateral armpit after hair removal; in group B, the concentration of subcutaneous injection was 5×107 in the lateral armpit after shaving with an animal shaver. After 0.2 mL of LM8 cell suspension of order of magnitude/mL was given daily local massage at the injection site, and the tumor growth was recorded respectively.

　　Result: The rate of A-composition tumor is 33% (4/12); the rate of B-composition tumor is 100%.

　　 Conclusion: Different injection techniques are of great significance to the tumor-bearing process of LM8 and the subsequent care for the tumor formation rate.