Establishment of tree shrew hand, foot and mouth disease model
动物模型,手足口病
z-bo
2022-02-03
472
Coxsackie virus A16 (CA16) is generally considered to be one of the main pathogens of human hand, mouth and oral diseases. The clinical manifestations of hand, foot and mouth disease include vesicles in the hands, feet, mouth, and severe inflammatory central nervous system damage. In this study, experimental CA16 was used to infect trees to study the cause of CA16.
Experimental animals: 15 healthy female sh (80±10g, 2 months) were randomly divided into infection group (n = 12) and mock infection control group (n = 3).
All experimental procedures have passed the management of laboratory animals in the Yunnan Office. All animals should be kept separately in accordance with the guidelines and policies of the Laboratory Animal Care Committee (CMAS) of the Institute of Biomedical Research, Chinese Academy of Medical Sciences. All individuals have been approved to pass the neutralization test, indicating the absence of CA16 antibodies. Experimental method: The experimental group jacks were infected with CA16 (104.5CCID50) from the respiratory tract through nasal spray, and the control group simulated a saline spray, which was a negative control without infection. The adolescents were monitored daily, and rectal temperature was measured with a digital thermometer stick (MCBOMR, Omron) two days after infection.
At the same time, EDTA-coated capillary venous blood samples were collected, and routine biological examinations were performed on the blood system of viral load (veterinary) species. Hemavet950FS, Drew Technology Co., Ltd.) All tissues were killed under anesthesia after the bluegrass was killed. On 4, 7, 10 and 14 days, the etiology and histopathology of the organs were examined. The results showed that the body temperature and the ratio of blood neutrophils and monocytes in sh infected with CA4 increased 4-7 days after infection, and each infection resulted in the dynamic distribution of CA16 in different tissues and feces.
It shows that it may be in a stage. Not only that, but pathological changes in the central nervous system and other organs can also be observed. This study shows that Mu sh can be used as an excellent animal model for the study of CA16 infection.
