绒毛膜癌移植瘤病理模型 z-bo 2020-12-01 406

　　Objective: To establish a stable and effective choriocarcinoma SCID beige mouse xenograft model, and observe the course characteristics and biological behavior of the model. Methods: 3~5 weeks old SCID beige female mice were randomly divided into experimental group and control group; 12 mice in the experimental group were divided into A (subcutaneous transplantation tumor model)/B (pulmonary metastasis model) two groups, respectively subcutaneously Injection or tail vein injection of 5×106 JAR cells per mouse, using morphology, radioimmunoassay to determine β-HCG, small animal in vivo imaging system (IVIS) and histological HE staining methods to identify JAR cell subcutaneous transplantation tumors and lung metastases Formation and so on. Results: 28 days after the experimental group was inoculated, mice in group A formed hard tumor nodules subcutaneously, and HE staining was consistent with the morphological manifestations of choriocarcinoma cells; mice in group B were detected by IVIS and showed single or multiple solid tumor masses in the body , HE staining showed that the lung tissues had tumor cell infiltration in varying degrees. On the 14th day of vaccination, the β-HCG level of the experimental group was significantly higher than that of the control group (P<0.05); the β-HCG level of group B was significantly higher than that of group A (P<0.05). Conclusion: SCID beige mice subcutaneously/tail vein injection of JAR cells can successfully construct a pathological model of human choriocarcinoma transplantation tumor. This model can simulate clinical choriocarcinoma epithelial solid tumor characteristics and lung metastasis characteristics, and is a study on the pathogenesis of human choriocarcinoma A good model of mechanism and experimental treatment.