肝损伤模型 z-bo 2020-12-02 499

　　Objective: To establish an Alb-cre/DTR double transgenic mouse model and analyze the related phenotypes. On this basis, to establish an inducible liver damage model for related research on liver diseases.

　　 Method: After the introduced Alb-cre and DTR mice are multiplied, double transgenic mice are obtained by crossing. Extract mouse tail tissue DNA, use PCR method for genotype identification. Diphtheria toxin was injected into the abdominal cavity of double transgenic mice, and then weighed and blood samples were taken at different time points to detect serum ALT and AST levels. Results: The Alb-cre and DTR mice were crossed and screened to obtain Alb-cre/DTR double transgenic mice. The use of 0.625 ng/g of diphtheria toxin in the mice can increase the serum ALT and AST levels. Significantly increased. After dissecting the mice, the whole liver was observed to turn white, and the HE staining results showed obvious necrosis of liver cells.

　　 Conclusion: Successfully established a mouse model that can induce specific liver injury.