酒精性肝损伤模型 z-bo 2020-12-02 438

　　Objective: To screen a simple, stable and reliable alcoholic liver injury model.

　　Method: Establish a mouse model of alcoholic liver injury by gavage with alcohol or give Lierber-DeCarli alcohol liquid feed for 8 weeks. During the experiment, the mental state, food intake and body weight changes of the mice were recorded, and the HE staining was performed for pathological analysis, and the serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyltransferase (γ-GT), and alkali were analyzed. Liver damage indicators such as AKP activity, serum and liver total cholesterol (TC) and triglyceride (TG) content.

　　 Results: After 8 weeks of modeling, both methods resulted in histopathological changes such as lipid accumulation in the liver of mice. Serum ALT, AST, AKP activities, serum and liver TG levels were significantly increased, suggesting significant liver damage. Alcohol gavage caused poor mental state of mice and significantly reduced body weight, while alcohol liquid diet had little effect on the mental state and weight of mice. The liver index and serum TC levels of the alcohol liquid feed model increased significantly, and the histopathological changes of serum ALT, AST activity, serum and liver TG content, and liver lipid accumulation were greater than those of the alcohol gavage model, suggesting that the former has a greater degree of liver damage than the latter The person is serious.

　　 Conclusion: The Lieber-DeCarli alcohol liquid feed model is better than the alcohol gavage model. The Lieber-DeCarli alcohol liquid feed model is more suitable for the study of the molecular mechanism of alcoholic liver injury and the screening of preventive drugs than the alcohol gavage model.