动物造模 z-bo 2022-11-14 455

　　[Modeling mechanism]: The theoretical basis of the anatomical injury model is the reentry theory of atrial flutter/atrial fibrillation, that is, the unidirectional circulation of one or more pulses in a closed loop. The model was established to prevent the surrounding tissues from conducting pulses and form a closed loop.

　　[Modeling method]: Select male and female mixed dogs, weighing about 20-35 kg, and intravenously anesthetized with sodium pentobarbital (30 mg/kg) for tracheal intubation and artificial ventilation. Open the chest through the fourth intercostal space on the right, incise the open cyst to fully expose the superior and inferior vena cava and the right atrium, and perform a surgical Y-shaped incision. The incision traverses the superior and inferior vena cava, and the Y-shaped collateral branch extends from the right atrium to the right atrial appendage. Then clamp the myocardium on both sides of the incision to form a linear incision suture, connecting the two incisions in a Y shape. Electrodes are placed on the surface of the atrium and trigger stimulation, causing atrial fibrillation/flutter.

　　[Model Features]: This model induces regular and frequent atrial arrhythmia after premenstrual contraction stimulation, and can be stably induced and stopped by pacing. It conforms to the characteristics of intraatrial reentrant tachycardia, similar to humans. The "incision" AF after congenital heart disease is very similar.

　　[Evaluation and Application of Model]: This model can be used for electrophysiology, pathology and treatment research of atrial fibrillation and flutter after cardiac surgery.