Hr基因敲除小鼠模型 z-bo 2020-12-03 437

　　Objective To establish knockout mice to study the function of hairless genes (Hr). Methods Using transcription activator-like effector nucleases (TALENs) technology to prepare Hr gene knockout mice, observe the hair growth and development of gene knockout mice, and take paraffin sections of skin tissues, microscope Analyze the results.

　　Result A first mouse with two base deletions at positions 86-87 in the coding region of Hr gene was obtained, and a TGA stop codon was generated. The positive offspring were obtained by crossing with wild-type mice, and they were mated in the same litter. The second generation mice began to lose their hair on the 14th day, and the hair was shed after 30 days, and remained hairless for life. Skin histological observation revealed that cysts of varying sizes were formed in the dermis. The results of the study showed that the successfully established Hr gene knockout mice showed abnormal hair growth.

　　 Conclusion It proves the close relationship between hairless genes and hair growth and development, and provides a good animal model for studying the precise functions of Hr genes.