2型糖尿病小鼠模型 z-bo 2022-01-13 371

　　Purpose: To compare the differences between spontaneous and induced type 2 diabetes mouse models in terms of glucose metabolism, insulin resistance and inflammation.

　　Method: Animals were divided into normal control group, induced model group and spontaneous model group, each with 10 animals. Normal control group: normal 11-week-old C57BL/6J mice; induced model group: use high-fat diet combined with STZ to induce 11-week-old C57BL/6J mice to establish a diabetes model; spontaneous model group: select 8-week-old C57BL/KsJ- db/db mice; monitor the changes in body weight, fasting blood glucose, and glucose tolerance of each group within eight weeks; detect changes in FINS, AUC, HOMA-IR, and TNF-α, IL-18, IL-1β, and INF-γ content before and after the experiment ; Eight weeks later, the animals were sacrificed to take liver, kidney, pancreas, testis, skeletal muscle and other tissues for pathological analysis. Results: During the experimental period, the body weight of the spontaneous model group continued to rise, while the body weight of the induced model group decreased significantly; the blood glucose of the induced model group decreased after eight weeks, while the spontaneous model group maintained high blood sugar stably; FINS increased, AUC and HOMA-IR decreased significantly. There was no significant change in FINS in the spontaneous model group, and both AUC and HOMA-IR increased significantly. At the beginning of the model establishment, serum pro-inflammatory factors (TNF-α, IL-18, The content of IL-1β and INF-γ) were higher than those of the normal control group. At the eighth week, the content of pro-inflammatory factors in the induced model group decreased, and the content of pro-inflammatory factors in the spontaneous model group increased significantly; the pancreas and other tissues of the two model mice had obvious inflammatory lesions. Conclusion: After eight weeks, the abnormal glucose metabolism and inflammation in the spontaneous type 2 diabetes model are gradually increasing, and the symptoms of hyperglycemia are maintained for a long time; the glucose metabolism and inflammation of the induced type 2 diabetes model are partially relieved after being fed for eight weeks, and the symptoms of hyperglycemia are partially relieved. Partly better.