哮喘模型小鼠 z-bo 2020-12-03 361

　　Objective: To observe the effect of pe glucoside on airway inflammation chemokines and their receptors in a mouse asthma model.

　　 Method: Use ovalbumin (OVA) to sensitize and challenge the establishment of a mouse asthma model. Serum IL-6, TNF-α levels and bronchoalveolar lavage fluid (bronchoalveolar lavage fluid, BALF) ELISA to detect ovalbumin-specific IgE (OVA)-IgE) and chemokine CCL19, CCL21 levels; chemotactic factors in lung tissue The chemical factor was tested by RT-PCR method to detect human CCR7 mRNA expression; Western blotting method was used to detect lung tissue CCR7 and nuclear factor-κB (nuclearfactor-κB, NF-κB) protein expression.

　　Results: The serum IL-6 and TNF-α levels of mice in the Pe drug intervention group were significantly reduced; BALF OVA-IgE, CCL19 and CCL21 levels were significantly reduced; the lung tissue CCR7 mRNA, CCR7 and NF-κB expressions were significantly reduced.

　　 Conclusion: Oni glucoside has a significant inhibitory effect on airway inflammation chemokine CCL19/CCL21 and its receptor CCR7 in asthma model mice.