裸鼠肺癌脑转移模型 z-bo 2021-12-23 429

　　Objective: To observe the ultrastructure of the blood-brain barrier in a lung cancer brain metastasis model in nude mice by using lanthanum nitrate tracer electron microscopy. Methods: Six BALB/c nude mice were randomly selected from the left ventricle and injected into the human lung adenocarcinoma cell PC-9 (1×106/0.1 mL) in the logarithmic phase as the model group; Two nude mice were treated as a blank group without any treatment. After the inoculation, the state of the nude mice was observed. At the 4th week, the ultrastructure of the blood-brain barrier was observed by lanthanum nitrate tracer electron microscopy, and the lungs and brain organs were collected for pathological HE staining.

　　Result: The nude mice in the model group began to lose weight at the 3rd week and gradually developed cachexia. All the nude mice were sacrificed at the 4th week. No metastases were seen in the thorax, lungs, brain, or organs when the specimens were taken by naked eyes. HE staining showed that the nude mice in the model group had multiple brain metastases of different sizes. Lanthanum nitrate tracer electron microscope showed that in the model group, lanthanum nitrate particles invaded outside the vascular cavity and distributed sparsely or diffusely in the brain tissue, while lanthanum nitrate particles in the blank group were only deposited in the vascular cavity.

　　 Conclusion: The dispersed lanthanum particles indicate the incompleteness of the blood-brain barrier structure in the model, and the occurrence of lung cancer brain metastasis is accompanied by the destruction of the blood-brain barrier structure.