慢性心力衰竭大鼠 z-bo 2020-12-03 351

　　OBJECTIVE: To establish a metabolic profile model of liver tissue in chronic heart failure rats, explore the changes in liver metabolism in chronic heart failure from a metabolic perspective, and explore characteristic metabolites that are valuable for the molecular mechanism of heart failure and disease diagnosis and treatment.

　　 Method: 20 Wistar male rats, the heart failure group underwent abdominal aortic constriction surgery, and the control group underwent sham surgery, and they were kept for 24 weeks. Metabonomics research platform was used to analyze the metabolic profile of rat liver tissue, and the orthogonal partial least squares discriminant model and principal component analysis model of heart failure rat liver tissue were established. Combined with the data processing of SPSS 19.0 software, the characteristic metabolites were finally obtained. Results: The principal component analysis model and the orthogonal partial least squares discriminant model were successfully established. At the same time, 10 characteristic metabolites that were different between the heart failure group and the control group were screened and identified from the model, including lysophosphatidylcholine, Lysophosphatidylethanolamine, oleic acid, glycocholic acid, dehydroepiandrosterone sulfate and other substances.

　　 Conclusion: The constructed metabolic profile model fits the metabolic disorder state of the liver in chronic heart failure rats well, and the selected characteristic metabolites can provide reference and help for the pathophysiological molecular mechanism of the disease and the diagnosis and treatment of the disease.