糖尿病模型 z-bo 2021-11-06 373

　　Objective: To construct a Beagle dog T2DM model with high-fat feeding combined with streptozotocin (STZ) and observe its related characteristics.

　　 Method: 30 ordinary male Beagle dogs were randomly divided into 3 groups: control group, high-fat group, diabetes model group, 10 in each group. The diabetes model group was fed high-fat feed and STZ was injected at the same time of feeding for 2 months; the high-fat group was fed high-fat feed; the control group was fed ordinary feed for 5 months. The animal's Lee index, fasting blood glucose, insulin, and urine glucose were measured on a regular basis, and the OGTT test was performed in March. After the test, blood lipids and other blood biochemical tests and liver and pancreas histopathological examinations were performed. Results: The Lee index increased significantly after high-fat feeding in the high-fat group and the diabetes model group for 2 months (P<0.01), and hyperinsulinemia and insulin resistance with dyslipidemia occurred; the fasting blood glucose of the diabetes model group increased significantly after STZ injection (P<0.01), 3="" and="" maintained="" high="" blood="" glucose="" levels="" for="" while="" the="" fasting="" of="" high-fat="" group="" did="" not="" change="" significantly="" p="">0.05), the diabetes model group OGTT test 3 h blood glucose value> 11.1mmol/L, and 3 h did not recover, and histopathological examination of the pancreas showed a certain degree of injury and disease. Conclusion: The high-fat diet induced insulin resistance and hyperlipidemia in Beagle dogs, but failed to form a hyperglycemic state. However, after high-fat diet combined with STZ injection, Beagle dogs developed insulin resistance at the same time as hyperlipidemia. The typical symptoms of T2DM with insulin resistance, such as hyperglycemia, hyperinsulinemia, and hyperlipidemia, are similar.