[Animal Modeling]-Vagus Atrial Fibrillation Model
[Modeling mechanism]: Studies have shown that the vagus nervous system may be involved in the formation and maintenance of atrial fibrillation, especially it is closely related to atrial fibrillation caused by pulmonary veins. The detailed mechanism is not yet clear, and may be related to the activation of acetylcholine-dependent potassium currents (IK, Ach) after vagus nerve stimulation, which shortens the atrial muscle action potential.
[Modeling method]: Choose an adult mixed dog. Fasting for 12 hours before the operation. After anesthesia, the trachea was intubated and the limbs of the electrocardiogram (ECG) were connected. Under the guidance of the X-ray machine, two 6F quadrupole electrodes were placed at the AV connection through the upper right chamber and bilateral femoral veins, and connected to the multi-channel physiological recorder. Disinfect the neck, trim the skin, and dissect both sides of the vagus nerve trunk from the neck. After the incision, the electrode is placed on the tip of the nerve heart and a constant pressure pulse with a pulse width of 0.1 ms is given. During continuous bilateral vagus nerve stimulation, rapid atrial pacing with a period of 90-120 ms induces atrial fibrillation.
[Characteristics of the model]: The success rate of this model is about 80%, and atrial fibrillation is durable and reproducible. After stopping the stimulation, the atrial fibrillation almost ended naturally.
[Model evaluation and application]: Vagus nerve stimulation + rapid atrial pacing atrial fibrillation model is relatively cheap (acute study), so it can provide continuous atrial fibrillation, and can provide vagus nerve stimulation according to the situation. This model is often used because it can stop atrial fibrillation, and preclinical studies are used to evaluate the effect of antiarrhythmic drugs on atrial fibrillation. It is also a fixed model for studying autonomic tension imbalance and paroxysmal atrial fibrillation.
动物造模
z-bo
2022-02-03
405