不同急性应激反应大鼠模型 z-bo 2020-12-03 306

　　Objective: To compare the changes of three acute stress response animal models and stress-related indicators, and seek to establish a relatively suitable acute stress response model.

　　 Method: 40 healthy adult male SD rats were randomly divided into normal group (I), tail docking group (II), burn group (III) and amputation group (IV), each with 10 rats. The normal group was not treated, and the tail shaft was cut and suspended near the tail end (between S3 and S4 spinal nerves), and the burn group was used as the body surface area of the 30% third degree burn model. Traumatic stress. Enzyme-linked immunosorbent assay was used to detect serum corticosteroid (ACTH) and corticosterone (CORT) levels in each group of rats, and immunohistochemical method was used to detect hypothalamic c-fos protein. The expression level is detected.

　　Result: Compared with group I, the levels of ACTH and CORT in groups II, III and IV increased.

　　 Conclusion: The acute stress response model created by cutting the low tail stem is characterized by simple operation process, more stable damage degree and obvious stress effect, which leads to unexpected accidents. It can reflect people's stress response more objectively. This is an ideal way to simulate an acute stress response.