阿霉素诱发肾性水肿病症 z-bo 2020-12-03 343

　　Objective: To investigate the feasibility of replicating a combined model of renal edema (deficiency of kidney yin and edema of kidney yang) in rats.

　　 Method: The rat kidney yin deficiency edema model was made by gavage of thyroxine tablets and tail vein injection of adriamycin, and the rat kidney yang edema model was made by intramuscular injection of hydrocortisone and tail vein injection of adriamycin. By observing the changes in physical signs of model rats, 24 h urine protein content, triiodothyronine (T3), thyroid (T4), cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP), estradiol (E2) ) And testosterone (T) and other indicators to determine whether the model is successful. Results: Animals in the yin deficiency and edema group showed clinical manifestations such as hyperactivity, hair loss, dry stool, weight loss, and body temperature increase. Compared with the blank group, T3, T4, cAMP, E2, and 24h urine protein levels were significantly increased, and cGMP and T levels were decreased. , The ratio of cAMP/cGMP increased significantly, and the differences in all indicators were significant (P<0.05). Animals in the Yang-deficiency and edema group showed signs of decreased activity, loose stools, weight and body temperature. Compared with the blank group, the contents of T3, T4, cAMP, and E2 were significantly reduced, and the contents of cGMP, T, and 24h urine protein were significantly increased. , The ratio of cAMP/cGMP decreased, and the difference of each index was significant (P<0.05).

　　 Conclusion: The combined animal disease model induced by thyroxine tablets combined with doxorubicin, hydrocortisone injection combined with doxorubicin is consistent with the diagnosis of kidney yin deficiency, yang deficiency and edema, and the modeling method is feasible.