胃癌原位移植模型 z-bo 2020-12-03 503

　　OBJECTIVE: To study the application effect of NIRF dye in in vivo imaging of orthotopic transplantation model of gastric cancer. Methods: Orthotopic transplantation of luciferin-labeled human gastric cancer cell line HepG2 into nude mice was used to establish a tumor model, and at the same time, a gastric ulcer model was induced; bioluminescence imaging and NIRF imaging were used for the above models to observe the effect of gastric cancer tissue on near-infrared fluorescent dye Absorption; explore the effects of hypoxia and anion transport peptide (OATP) on the absorption of NIRF dyes by gastric cancer tissues, and clarify the specificity of NIRF dyes to target and recognize tumor cells.

　　Result: NIRF signal and bioluminescence signal have a good correlation in the in vivo imaging of orthotopic transplantation model of gastric cancer. Strong NIRF fluorescence signal can be obtained in gastric cancer tissue, but no fluorescence signal can be detected in gastric ulcer. Hypoxia can enhance the absorption of NIRF dye by gastric cancer cells, and the anion transit peptide specific inhibitor sulphobromide sodium (BSP) can significantly reduce the absorption of NIRF dye by tumor cells.

　　 Conclusion: Heptamethine cyanine near-infrared fluorescent dye can target and identify the orthotopic transplantation model of gastric cancer.