高脂血症模型 z-bo 2020-12-03 660

　　Objective: To feed SD rats with a high-fat diet for 8 weeks to establish hyperlipidemia, to verify the feasibility of this modeling method, and to explore the changes in blood lipids of rats at different time points.

　　 Method: 30 normal male SD rats of 8 weeks old, after 1 week of adaptive feeding, were randomly divided into control group, model 1 group, and model 2 groups according to their body weight, each with 10 rats. The control group was given normal feed, and the model group 2 was fed high-fat and high-cholesterol feed for 8 weeks. The diet and drinking water of each group were measured daily, and the litter was changed and weighed for 4 days. At the 4th week, 6th week and 8th week respectively, the animals were fasted overnight and blood was collected from the orbital venous plexus to determine the four levels of blood lipids in each group. The animals were sacrificed at the end of the 8th week, and the liver and aorta of each group were collected for HE staining.

　　Result: Compared with the control group, the average daily dietary intake of the model 1 group increased, but the difference was not statistically significant; the daily average dietary intake of the model 2 group decreased significantly, and the difference was statistically significant (P<0.01). Compared with the control group, the daily water consumption of the model group 2 groups was significantly reduced, and the difference was statistically significant (P<0.01). Compared with the control group, the liver/body mass index of the model group increased significantly, and the difference was statistically significant (P<0.01). Compared with the control group, the final body weight of the model group 2 increased, but only the model 1 group had statistical significance (P<0.05). In terms of blood lipids, compared with the control group, the TC levels of the 2 groups in the 4 and 6 weekend model groups were significantly increased, and the difference was statistically significant (P<0.01 or P<0.05). The LDL-c level in the 8 weekend model group 1 was significantly increased High, the level of HDL-c decreases, and the difference is statistically significant (P<0.05). The HE staining of the liver showed that the liver cells in the control group were arranged normally and stained evenly, while the liver cells in the model group 2 had a large area of fatty degeneration, and a few liver sinusoids were congested and inflammatory cell infiltration. HE staining of aorta showed normal aortic structure in 3 groups.

　　Conclusion: SD rats fed with high-fat diet to establish hyperlipidemia are established, and the model created is hypercholesterolemia with severe fatty liver. At the same time, during the experiment of hyperlipidemia in SD rats fed with high-fat and high-cholesterol feed, the fluctuation of blood lipids showed a state of increased-internal adaptation-increased. In the process of modeling, how to overcome the problem of animal anorexia and internal regulation of cholesterol metabolism is the key to high-fat feed feeding.