阿尔茨海默病转基因模型 z-bo 2020-12-04 319

　　Purpose: The pathological changes of Tg2576 transgenic mice are similar to those of Alzheimer's disease (AD) patients. This article dynamically studies the characteristics of serum metabolites in Tg2576 mice at different stages of AD, and provides metabolism for early diagnosis of clinical AD in accordance with.

　　 Method: Collect the serum samples of Tg2576 mice at the initial stage (6 months) and the final stage (12 months) of AD onset, collect the 1HNMR spectrum of the samples and use multivariate analysis methods to analyze the metabolic characteristics.

　　Results: The results showed that the serum metabolic characteristics of Tg2576 and C57 mice were significantly different at 6 and 12 months, respectively, and Tg2576 mice at different stages of AD had obvious metabolic differences. Compared with C57 mice, in the early stage of AD appearance, the serum levels of lactic acid, inositol and amino acids (such as leucine, isoleucine, and alanine) in Tg2576 mice increased, while lipids and choline The content of phosphatidylcholine/glycerol phosphatidylcholine, betaine, glycine and glucose decreased; at the end of the onset of AD, the content of serum lactic acid, inositol and alanine continued to rise, lipids, choline, and phosphatidylcholine The content of alkali/glycerol phosphatidylcholine, betaine and glycine continued to decrease, while the content of glutamate and creatine initially showed a downward trend. By comparing the early and end-stage serum metabolites of AD, we can find that serum lactic acid, inositol and alanine levels increase, and lipid, choline, phosphatidylcholine, and glycerophosphatidylcholine levels decrease in the end-stage disease. Among these metabolites, lactic acid, lipid, choline, phosphatidylcholine and glycerol phosphatidylcholine have significant changes in the early stage of AD, and are closely related to the severity of AD. Conclusion: The results showed that lactic acid in Tg2576 mice was positively correlated with the severity of Alzheimer’s disease, while lipid, choline, phosphatidylcholine and glycerol phosphatidylcholine were negatively correlated, and these metabolites The development of the disease shows dynamic and progressive changes, which may be an important metabolic marker for early diagnosis of AD.