肝郁证动物模型 z-bo 2020-12-04 364

　　Purpose: To compare the effects of single emotional stimulation and compound emotional stimulation on animal models of liver depression syndrome, in order to screen out a modelling method that is closer to the clinical manifestations of liver depression syndrome.

　　 Method: Through the preliminary screening, a single model model of the tail-clamping method, the limb binding method and the neck yoke method and the three-method composite model were established respectively. Establish a syndrome comparison table between liver depression syndrome and rat syndrome and liver depression syndrome rat syndrome classification table, combining TCM syndrome indicators, general observations, body weight indicators, positive drug counter-syndrome and determination of hormone indicators in the body to verify the modeling results . Results: The rats in the normal group grew well; the rats in the model group mainly showed acute stress mania and chronic stress depression, with dry, few, and small stools. Most of the rats had dark red eyes, hair loss, and shortness of breath. Rats The weight growth rate decreased, the hypothalamic CRH levels, plasma CORT, and ACTH levels were significantly higher than those of the normal control group, and the effect of the composite model was significantly better than the single model; the positive drug group had symptoms after drug treatment Significantly improved, and the indicators also approached the normal group. Conclusion: The use of tail-clamping, limb binding and neck yoke compound emotion modeling method to construct a rat model of liver depression syndrome has a short cycle, good stability, and high success rate. The animal performance and clinical liver depression syndrome are higher. Consistency.