慢性心肌缺血模型 z-bo 2020-12-04 352

　　In view of the shortcomings of the previous pig chronic myocardial ischemia model, this study first used the mechanism of hyperlipidemia to cause AS and cause coronary artery obstruction or stenosis, and adopted a high-fat diet to cause chronic myocardial ischemia in pigs. Supplement injection strengthens the sensitivity of myocardial ischemia, simulates the induction of clinical sudden coronary heart disease, and establishes a miniature pig chronic myocardial ischemia model. This research model avoids surgical damage, is similar to clinical occurrence, has good stability and reproducibility, and has few influencing factors. It is more suitable for coronary heart disease prevention and treatment research and new drug pharmacodynamic evaluation research. At the same time, non-invasive telemetry technology is used to detect and evaluate the symptoms of myocardial ischemia models, and to perform long-term continuous dynamic electrocardiogram detection and heart rate variability analysis in a awake state, which can reflect the occurrence and development of pathological changes in model animals to a certain extent , And combined with myocardial enzymes, blood lipids, free radicals, myocardial energy metabolism, blood cohesion and electrolyte indicators and other blood biochemical indicators for auxiliary evaluation research, to provide a basis for the establishment of evaluation technical specifications for miniature pig chronic myocardial ischemia model. Considering that some domestic mini-pigs may have particularities in the application of cardiovascular diseases, this study purposefully selected Wuzhishan mini-pigs, Bama mini-pigs, and Tibet mini-pigs, and discussed the occurrence of hyperlipidemia, hypertension, and hypertension in these three mini-pigs. Different characteristics of AS risk factors such as hyperinsulinemia. The non-invasive telemetry technology combined with blood biochemical indicators is used to evaluate the transportation stress response of these three types of miniature pigs, and define the adaptation and recovery period of miniature pigs after transportation stress, which provides a basis for the practical application of miniature pigs in the field of biomedicine.