大鼠动脉移植模型 z-bo 2020-12-04 336

　　Purpose: Allograft vasculopathy (AV) limits the function and long-term survival of transplanted organs. It is the most urgent worldwide problem in the field of organ transplantation. Traditional vascular suture methods require excellent microsurgery techniques and are difficult to carry out. Exploring a more convenient and feasible arterial transplantation method can provide ideas for clinical research on AV. Methods: A self-made vascular cannula was used with a venous indwelling needle. The thoracic aorta of the donor rat was pulled into the cannula, both ends were turned over, ligated and fixed into a graft. The graft was implanted in the abdominal aorta of the recipient rat One week, four weeks, and eight weeks after modeling, the grafted arteries were taken for HE staining to observe the morphology of grafted vessels and measure the intimal thickness. Results: Among 40 recipient rats, 38 survived to the required detection time point. The lower limbs and tail moved freely, and urine and feces were normal, and HE staining showed that the grafted vascular intima showed a concentric circle-like increase consistent with AV. thick.

　　Conclusion: This rat arterial transplantation method is simple and feasible. Compared with the traditional vascular suture method, it can significantly shorten the occlusion time of the abdominal aorta, reduce the surgical trauma of the recipient rat, and has a high success rate of modeling and strong reproducibility.