哮喘小鼠模型 z-bo 2020-12-04 313

　　Objective: To observe the role of interleukin 22 (IL-22) in asthma models, and to study the effect of budesonide on airway inflammation and IL-22 regulation in asthma mouse models.

　　 Method: 24 BALB/c mice were randomly divided into three groups: control group, asthma model group and budesonide group. The mice were sensitized and challenged with ovalbumin (OVA) to prepare asthma models. Mouse lung tissues were stained with HE and AB-PAS to score airway inflammation. ELISA method was used to detect the level of IL-22 in mouse alveolar lavage fluid (BALF), and real-time fluorescent quantitative PCR was used to detect IL in mouse lung tissue. -22mRNA expression level.

　　 Results: Compared with the control group, the lung tissue inflammation score of asthmatic mice increased, the level of IL-22 in BALF increased, and the level of IL-22mRNA expression in lung tissue increased. The difference was statistically significant. After treatment with budesonide, the airway inflammation score and the expression of IL-22mRNA in lung tissue of the mice were lower than those in the asthma group, and the difference was statistically significant.

　　 Conclusion: The therapeutic effect of budesonide on asthmatic airway inflammation is related to its inhibition of the expression and secretion of IL-22 in the lung.