糖皮质激素肾阴虚肾阳虚证模型 z-bo 2020-12-04 369

　　Purpose: To observe the role of resting metabolic rate (RMR) in evaluating the glucocorticoid model of kidney-yin deficiency and kidney-yang deficiency.

　　Methods: BALB/c male mice were divided into control group, model group, Jinkuishenqi pill group, and Zhibaidihuang pill group. The last three groups were given drinking water containing corticosterone, and the control group was given 1% ethanol Drinking water. A closed fluid pressure respirometer was used to determine the animal RMR. At the end of the experiment, the mice were sacrificed, and the weight index of perinephric fat, epididymal fat, quadriceps femoris, and tibial anterior muscle were detected; serum hormone levels were detected by ELISA; HE staining of liver and kidney tissues were observed; , MDA) content, succinate dehydrogenase (SDH) activity, cytochrome-c-oxidase (COX) activity, ATP level.

　　Results: Compared with the control group, the energy metabolism rate of the model group increased significantly on the second day of the experiment, reached the highest on the fourth day (P<0.01), then gradually decreased, and significantly decreased on the 66th day (P<0.05). Corticosterone modelling for 66 days significantly reduced the serum thyroxine (T4) content, increased fat mass index, decreased muscle mass index, increased MDA level, inhibited SDH, COX activity and ATP level. Both kidney-tonifying drugs can reduce animal mortality and liver tissue MDA content, and Jinkuishenqi Pill can increase the energy metabolism rate on the 4th and 66th day of the model group (P<0.05), and can significantly increase the SDH activity of liver tissue , COX activity and ATP level (P<0.01), similar effects were not observed in Zhibai Dihuang Wan.

　　Conclusion: The resting energy metabolism rate has a certain effect on the evaluation of glucocorticoid kidney yin deficiency/kidney yang deficiency syndrome model.