Ⅱ型糖尿病大鼠 z-bo 2020-12-04 331

　　Objective: To establish a model of exercise to improve glucose metabolism in type 2 diabetic rats, and to provide a theoretical reference for the establishment of exercise prescription for type 2 diabetes. Methods: Forty-five SPF male Wistar rats aged 8 weeks, 32 were randomly selected to be fed with high-sugar and high-fat diet for 7 weeks and injected intraperitoneally with a small dose of streptozotocin (STZ) buffer to establish type II diabetes. Rat model. Normal rats and successfully modeled rats were divided into 4 groups: blank control group (C group), simple exercise group (CE group), diabetes control group (DM group), and diabetic exercise group (DME group). The exercise group used an improved Ploug training program, 60 min/d, 6 days a week, a total of 8 weeks of training. After 7 weeks of high-fat diet, blood was collected from the tail vein to determine fasting blood glucose and serum insulin. At baseline and 8 weeks after exercise, blood was collected from tail vein to determine fasting blood glucose (FBG), and blood was collected after 8 weeks of exercise to determine serum insulin. (FINS), and calculate the insulin resistance index (HOMA-IR).

　　Result: ①After 7 weeks of high-sugar and high-fat feeding, the content of FBG, FINS and HOMA-IR in the high-fat group was significantly higher than that of the normal group. ②After 8 weeks of exercise intervention, the FINS levels in the DM and DME groups were significantly lower than those in the C and CE groups, and FBG and HOMA-IR were significantly higher than in the C and CE groups; the FINS levels in the DME group were significantly higher than those in the DM group, FBG and HOMA- IR was significantly lower than the DM group. The body weight of DM group and DME group was significantly lower than that of C group and CE group; there was no significant difference in body weight between DME group and DM group, CE group and C group. Conclusion: ①The 7-week high-sugar and high-fat feeding combined with one-time intraperitoneal injection of STZ (30 mg/kg) successfully established the type 2 diabetic rat model; ② The swimming exercise pattern of 60 min/d for 8 weeks can improve the glucose of type 2 diabetic rats Metabolism is an ideal animal model for studying the mechanism of exercise prevention and improvement of diabetes.