兔变应性鼻炎模型 z-bo 2020-12-04 432

　　OBJECTIVE: To compare and observe the dendritic cells (dendritic) from peripheral blood of white-haired black-eyed rabbits (WHBE rabbits) and Japanese big-eared white rabbits (JW rabbits) induced by ovalbumin (OVA) in allergic rhinitis (AR) models. The function of cells (DC) reveals the possible mechanism of WHBE rabbit sensitivity to AR.

　　 Method: WHBE rabbit and JW rabbit AR models were established by OVA induction. After each challenge, the general signs of the animals were observed, the nasal mucosa was stained with HE, and the histopathological changes were observed. Peripheral blood DCs were isolated, and flow cytometry was used to detect the expression of the surface molecule CD86 on peripheral blood DCs and the ability of DCs to take up antigens. At the same time, fluorescence quantitative PCR was used to detect the expression level of mannose receptor (MR) mRNA in peripheral blood DCs. T cells were further labeled with CFSE, and the ability of peripheral blood DC to induce T cell proliferation was detected by flow cytometry.

　　Results: General physical signs and HE staining observations showed that the animals in the WHBE rabbit and JW rabbit model groups showed typical allergic rhinitis symptoms and histopathological changes, suggesting that the model was successfully constructed. The expression of DC CD86 in peripheral blood of WHBE rabbit AR model group was not only extremely significantly higher than that of normal control group, but also extremely significantly higher than that of JW rabbit AR model group (P<0.01, P<0.01). Under normal steady state, the relative expression of DC MR mRNA in the peripheral blood of WHBE rabbits was significantly higher than that of JW rabbits (P<0.01). The AR model was established by OVA induction, and the mRNA expression level of DC MR in the peripheral blood of WHBE rabbits increased significantly (P <0.05), and was significantly higher than the JW rabbit model group (P<0.05). In addition, the ability of peripheral blood DC to take OVA647 in the WHBE rabbit model group was significantly higher than that of the normal control group (P<0.01), and also significantly higher than that of the JW rabbit model group (P<0.01).

　　 Conclusion: WHBE rabbit DC is more sensitive to allergens, which may be related to its high expression of the antigen recognition receptor MR, stronger differentiation and maturity, and antigen uptake ability.