小鼠肝癌模型 z-bo 2020-12-04 496

　　Objective: To establish a mouse liver cancer model induced by diethylnitrosamine (DEN) and use two different formula feeds: SPF-grade mouse and rat complete compound feed and AIN93-G formula synthetic feed separately to explore different feeds Influence on the construction of DEN-induced mouse liver cancer model.

　　 Method: 14-day-old male C57BL/6 mice were injected intraperitoneally with DEN solution once at a dose of 25 mg/kg body weight to establish a mouse liver cancer model. After weaning, the mice were divided into two groups. One group was fed with regular SPF-grade full price compound feed for rats and mice, and the other group was fed with AIN-93G formula synthetic feed. The model mice were killed when they were 9 months old, and the liver tissues were taken and weighed. The growth of liver cancer was observed and recorded. Results: All mice in the complete diet group developed tumors. The body weight and liver weight of the mice in the AIN-93G group were significantly lower than those in the complete diet group. The incidence of liver cancer and the number and size of tumor nodules were also significantly lower than those in the complete diet. Group mice. Conclusion: Mice fed with complete diets successfully induced liver cancer model with DEN, while mice fed with AIN-93G diet inhibited the occurrence and development of liver cancer. At the same time, the weight of mice decreased significantly, suggesting the establishment of dietary factors in animal disease models. Play a key role in