宫内发育迟缓模 z-bo 2021-11-25 348

　　Purpose: Intrauterine growth retardation (IUGR) is manifested in the form of low birth weight of newborns? The IUGR model is established by three methods commonly used in experiments, and through the comparison and analysis of experimental data, it is the IUGR model in scientific research. Provide a reference basis for the establishment of? Methods: Twenty pregnant mice on the 0th day of pregnancy were randomly divided into four groups, 5 in each group. Three of them were treated with alcohol intervention (A), uterine artery ligation (U), and low protein diet (LP). The IUGR rat model was established by other methods. The fourth group was a normal control group (CON). Fetus rats were taken by cesarean section on the 20th day of pregnancy, and the fetal rats were weighed for their body mass, brain mass, placental mass, and kidney mass. Wait, and measure the body length and tail length, calculate the incidence of IUGR, survival rate and specific gravity of the brain, specific gravity of the placenta, etc.; each group randomly continued to raise the fetuses of cesarean section, and measure their body at 3 weeks, 6 weeks, and 12 weeks. Mass? Perrenal fat mass and some organ masses? Results: In the alcohol intervention method, the uterine artery ligation method, and the low-protein diet method, the average body weight of the three groups of fetal mice was significantly lower than that of the normal control group (P<0.05), and the incidence of fetal IUGR was significantly higher than that of the normal control group (P <0.05). 0.05), the survival rate of fetal rats in the uterine artery ligation group was lower than that of the other two groups. After the rats were fed for 3 weeks, the average body weight of the rats in the experimental group was significantly lower than the normal control group (P <0.05); 6="" after="" weeks="" of="" the="" experimental="" group="" average="" body="" weight="" rats="" in="" was="" still="" lower="" than="" that="" normal="" control="" and="" difference="" not="" significant="" p="">0.05); after 12 weeks of feeding, the average body weight of rats in the alcohol intervention group and the low-protein diet group was significantly higher than that of the normal control group ( P<0.05)? Conclusion: All three modeling methods can successfully establish the IUGR model. Among them, the IUGR animal model established by the low-protein diet group has a high incidence and a low stillbirth rate, which is suitable for model research; in terms of catch-up growth, the alcohol intervention group and low protein The average body weight of the diet group during the postpartum and lactation period of the cesarean section rats was lower than that of the normal control group, but the growth rate after the lactation period was much higher than that of the normal control group, indicating that these two experimental groups have caught-up growth?