慢性肾脏病大鼠模型 z-bo 2020-12-06 364

　　Objective: To observe the characteristics of mineral metabolism biochemical markers in adenine-induced chronic kidney disease (CKD) rat model? Vascular calcification and renal bone disease changes?

　　Methods: Twenty male SD rats were randomly divided into 2 groups: normal control group and CKD group? At the second weekend, serum biochemical markers were detected? At the 6th weekend, rats were sacrificed and serum biochemical markers were detected? Aortic vascular pathology Inspection and determination of vascular calcium and phosphorus content, take femur and fifth lumbar vertebrae for bone mineral density (BMD) examination? Bone morphometric analysis? Results: At the 2nd and 6th weekends, the blood creatinine, urea nitrogen, blood phosphorus, and serum parathyroid hormone of the CKD group were significantly higher than those in the normal control group, and the blood calcium was significantly decreased. 50% of the rats in the CKD group Median vascular calcification occurred, and no vascular calcification occurred in the normal control group. The vascular calcium and phosphorus content of the CKD group was significantly greater than that of the normal control group. BMD examination. Compared with the normal control group, the total femur, femoral cortical bone, and femoral bone of the CKD group were small. The BMD of the beam bone and the fifth lumbar vertebra were significantly reduced? In terms of bone morphometry, the trabecular bone resorption and bone formation of the CKD group were at a high level and were in a high conversion state; the trabecular bone and cortical bone of the CKD group The bone mass is significantly lower than that of the normal control group; there is no significant difference in trabecular bone mineralization between the CKD group and the normal control group?

　　Conclusion: The adenine-induced CKD rat model showed hypocalcemia, high blood phosphorus and high serum parathyroid hormone; vascular calcification showed media calcification; renal osteopathy showed high trabecular bone turnover? normal mineralization And low cortical bone and trabecular bone bone mass are also in line with the characteristics of fibrous osteitis? Can adenine-induced chronic kidney disease rat model be a good carrier for future research on bone and mineral metabolism abnormalities in chronic kidney disease?