大鼠腰椎腹侧神经根牵拉模 z-bo 2020-12-07 337

　　Purpose: In order to study the pathogenic mechanism and drug development of amyotrophic lateral sclerosis, establish the 4th lumbar ventral nerve root traction rat model, and use brain-derived neurotrophic factor as a positive drug to verify the effectiveness of the model? Methods: First, 5 male SD rats were operated on. One week later, immunohistochemical staining was performed with anti-choline acetyltransferase antibody to observe the changes in the number of motor neurons in the anterior horn of the spinal cord. 7-week-old SD male rats were randomly divided into four groups, two groups of model control group and two brain-derived neurotrophic factor treatment groups (prophylactic administration group immediately after surgery and therapeutic administration group one week after surgery). How does anti-choline acetyltransferase immunohistochemical staining observe the changes in the number of motor neurons? Results: The rats recovered well after the operation, and there were no abnormalities in the clinical observations. The staining results proved that the anterior horn of the spinal cord was degeneration and death of motor neurons after the operation. Compared with the control group, the nerves treated with brain-derived neurotrophic factor Root pulling animals, whether it is prophylactic administration or therapeutic administration 1 week after surgery, has achieved good therapeutic effects, and the number of choline acetyltransferase staining positive neuronal cells increased significantly (P<0.0001), the result They are 17.85% to 93.06%; 26.6% to 87.27%?

　　 Conclusion: The successful establishment of a traction model of the 4th lumbar ventral nerve root in rats provides a valuable animal model for the study of amyotrophic lateral sclerosis?