肾缺血再灌注损伤模型 z-bo 2020-12-07 353

　　Objective: To explore a method for making a rat renal ischemia-reperfusion injury model with little damage, simple operation and stable effect.

　　 Method: SD male rats were randomly divided into normal group (C group), sham operation group (S group), and experimental group (IR group). The rats in the experimental group entered the bilateral retroperitoneal space through the back muscle fascia through the median skin incision on the back, separated the bilateral renal pedicles, clamped the bilateral renal pedicles with non-damaged micro-arterial clamps for 50 minutes, and then released them to recover. Perfusion. Rats in the sham operation group did not clamp the renal pedicles, and the remaining steps were the same as those in the experimental group; rats in the normal group were only anesthetized. The survival status, blood creatinine, urea nitrogen and renal tissue structure changes were observed 24 hours after operation. Results: In the IR group, the skin incision was (2° 24 ± 0° 27) cm, the right lumbar back fascia and muscle cut (1° 36 ± 0° 21) cm, the left lumbar back fascia and muscle cut (1° 36 ± 0.24) cm. From incision of the skin to clamping the pedicles of the kidneys, it takes (3 30 30 ± 37) min. The IR group is modeled into a power of 95%. Compared with the normal group and the sham operation group, the blood creatinine and blood urea nitrogen levels in the IR group were significantly increased (P ＜ ΟΤ 01), and the renal tubule injury score was significantly increased (P ＜ ０05)

　　 Conclusion: The use of back-entry retroperitoneal double kidney pedicle clamping method to establish a renal ischemia-reperfusion injury model has stable effect, high success rate, small incision, less bleeding, light irritation to animals, and easy operation.