胰腺癌原位移植模型 z-bo 2020-12-07 346

　　Objective: To establish an orthotopic transplantation model of pancreatic cancer based on clinical tumor specimens, and to carry out model evaluation research. Methods: Transplanted clinically fresh surgical specimens of pancreatic cancer to the pancreas of nude mice, established orthotopic xenotransplantation model (PDOX), assessed the tumor growth by in vivo imaging technology and PET/CT; analyzed the source of transplanted tumors by STR technology To determine the pathological characteristics of transplanted tumors through histomorphological observation and immunohistochemistry, and to detect the expression level of CA19-9 in the peripheral blood of the PDOX model. Results: Six weeks after the model was established, the near-infrared fluorescence signal was observed to be significantly enriched in the tumor site through in vivo imaging, and the tumor location and size can be preliminarily judged by the fluorescence intensity; using small animal PET/CT can clearly observe the abdomen 18 F-FDG The molecular probe enrichment area is consistent with the expected tumor location and size; after euthanasia, the separated organs and tumor tissues are dissected, and the tumor growth status is further confirmed by near-infrared fluorescence imaging; STR testing confirms that the transplanted tumor is human The sex ratio was 99%. Histomorphology and immunohistochemical analysis showed that the transplanted tumor grew on the pancreas of nude mice. The CA19-9 detection in serum further confirmed the occurrence of pancreatic cancer.

　　 Conclusion: A human pancreatic cancer orthotopic xenotransplantation model was successfully established. The model was comprehensively evaluated through small animal in vivo imaging, PET/CT and other technologies, which provided a good animal model for the pathogenesis and treatment of pancreatic cancer.