链脲佐菌素诱导糖尿病大鼠模型 z-bo 2020-12-07 321

　　Objective: To observe the changes in the structure and abundance of the intestinal flora in a diabetic rat model induced by streptozotocin (STZ).

　　 Method: 25 male SD rats were randomly divided into control group (C) 10 rats and model group (M) 15 rats. The model group was intraperitoneally injected with 3% STZ at a dose of 30 mg/(kg·bw) for 5 days. After forming the model, measure the weight, blood sugar and other indicators every month. When the model was established at 4 and 12 weeks, fresh rectal feces from each group of rats were taken at the same time. The 16S rDNA-V3 region of bacteria in stool samples was sequenced on the Illumina high-throughput sequencing platform, and the structure and abundance of the intestinal flora were quantitatively analyzed.

　　Result: There is no significant difference in the number of optimized sequences obtained between the model group and the control group (P> 0.05). The relative abundance index (Chao1) and diversity index Shannon of the intestinal flora of the model group were lower than those of the control group (P<0.05), and the Simpson index was higher than that of the control group (P<0.05). At the phylum level, the relative abundance of Proteobacteria, Cyanobacteria, Tenericutes, TM7, Actinobacter (Actinobacter) decreased (P<0). At the genus level, the relative abundance of Lactobacillus (Lactobacillus) in the model group decreased (P <0.05); the relative abundance of Bacteroides (Bacteroides) increased (P <0.05). After 12 weeks of modeling, the relative abundance of Lactobacillus (Lactobacillus), Bacteroides (Bacteroides), and Ruminococcus (Ruminococcus) in the model group increased (P <0.05); the relative abundance of Bifidobacteria (Bifidobacter) Decrease (P <0.05).

　　 Conclusion: The abundance and diversity of the intestinal flora of diabetic animals in SD rats induced by STZ decreased, which provides a reference for the study of the relationship between diabetes and intestinal flora.