口腔黏膜炎模型,5-氟尿嘧啶 z-bo 2020-12-08 369

　　Objective: To establish a model of oral mucositis in golden hamsters caused by 5-fluorouracil combined with mechanical scratches. Methods: Male golden hamsters were divided into a control group and a model group. The animals in the model group were intraperitoneally injected with 60 mg/kg and 40 mg/kg 5-fluorouracil (5FU) on day 1 and day 2, respectively, on day 4 The right cheek pouch of the golden hamster was turned out, and an 18-gauge needle was used to form a superficial oral scratch in a linear scratch. The control animals were injected with an equal volume of normal saline on the first day and the second day. After that, observe the macroscopic changes of the right cheek pouch of each group every day and take pictures to score, and record the weight and the average daily food intake of each animal. Blood was taken on the 8th and 14th days after the model was established to determine the pro-inflammatory cytokine TNF in the serum. -α and IL-1β content, take the right cheek pouch tissue for HE staining and measure the malondialdehyde (MDA) content and superoxide dismutase (SOD) activity. Results: Compared with the control group, the golden hamster in the model group had an ulcer on the right cheek pouch tissue on the 3rd day after modeling and had the highest macroscopic score. Within 8 days after modeling, the average daily food intake and weight loss of the animals were significantly reduced. On the 8th and 14th days after the model, the golden hamster’s right cheek pouch tissue structure was damaged, showing an inflammatory reaction, the serum TNF-α and IL-1β levels were significantly increased, and the MDA content in the cheek pouch tissue was significantly increased. High, SOD activity is significantly reduced.

　　 Conclusion: 5-Fluorouracil combined with mechanical scratching can lead to the formation of golden hamster oral mucositis. The inflammatory reaction is obvious within 8 days after modeling, and it is most obvious on the third day. It can be used to study the pathogenesis of oral mucositis and therapeutic drugs.