关节炎大鼠模型 z-bo 2021-11-11 399

　　Objective: To investigate the effect of melittin intervention on the regulation of Th17/Treg balance and joint inflammation in a rat model of collagen-induced arthritis. Methods: 30 groups of SD rats were randomly divided into 6 normal control groups. The remaining 24 SD rats were injected with bovine II collagen at the base of the tail on day 1, and collagen II was injected again on day 10 to establish a collagen-induced arthritis model. 6 unsuccessful modeling rats were eliminated every day, and 18 successful modeling rats were selected according to the random number table method and divided into model group, melittin group, methotrexate group, 6 rats in each group. 14 Melittin group was intraperitoneally injected with melittin (0.5 mg/kg, once every other day) in the perivenomin group, methotrexate was injected in the methotrexate injection group (1 mg each, once a week, 3 times in total), model group, The normal group was intraperitoneally injected with the same amount of sterile water for injection. On the 32nd day after anesthesia, the serum was collected, and the concentration of TNF-α, IL-10, IL-17A in the serum was detected by ELISA, the spleen of the rat was separated, the lymphocytes were extracted, and the MTS was detected. Mononuclear cell activity, flow cytometry to detect the proportion of Th17 and Treg cells, HE staining to observe the pathological changes of the ankle joint. Results: Melitin and methotrexate reduced the concentration of TNF-α and IL-17A in serum (P<0.0 P<0.05), and increased the concentration of IL-10 (P<0.05); Melitin and methotrexate Inhibition of lymphocyte activity (P<0.05); Melitin and methotrexate can down-regulate the proportion of Th17 cells (P<0.05) and up-regulate the proportion of Treg cells (P<0.0 P<0.05); Melitin and methotrexate The group reduced synovial hyperplasia, inhibited inflammatory cell infiltration, and delayed bone and articular cartilage destruction.

　　 Conclusion: Melittin can regulate the collagen-induced Th17/Treg balance in the rat model of joints and inhibit joint inflammation.