心肌缺血,动脉粥样硬化斑块 z-bo 2020-12-08 312

　　Objective: To explore an effective method to establish a dual target mouse model of myocardial ischemia and atherosclerosis (atherosclerosis, AS) plaque angiogenesis. Methods: 10 C57BL/6J mice were used as the control group, and 10 ApoE-/- mice were used as the model group. The control group was fed with ordinary feed, and the model group was fed with high-fat feed. The model group was fed with high-fat feed for 8 weeks and then injected subcutaneously Isoproterenol 100mg/kg, continuous 2d, the control group was injected with the same amount of saline subcutaneously. 4 weeks later, the serum total cholesterol (total cholesterol, TC), triglyceride (triglyceride, TG), low-density lipoprotein cholesterol ( Lowdensity lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) levels; HE staining to observe the pathomorphological changes of the aorta and myocardium; CD31 immunohistochemical staining to detect the aorta Density of new blood vessels in different parts of myocardial tissue. Results: The blood TC and LDL-C levels of the model group were significantly higher than those of the control group (P<0.05), while HDL-C was significantly lower than the control group (P<0.05); HE staining showed that the model group’s aorta formed a typical AS disease Physical changes; 4 weeks after subcutaneous injection of isoproterenol, HE staining of myocardial tissue in the model group showed typical pathological changes of myocardial infarction; CD31 immunohistochemical staining results showed that the density of aortic neovascularization in the model group was significantly higher than that in the control group (P< 0.05), the expression of neovascularization in the myocardial ischemic area of the model group was the most abundant, which was significantly higher than the myocardial infarction area and normal myocardial tissue area (P<0.05).

　　Conclusion: ApoE-/- mice under a high-fat diet, through subcutaneous injection of isoproterenol to cause myocardial infarction, can successfully establish a dual-target mouse model of myocardial ischemia and AS plaque angiogenesis.