气滞血瘀型慢性输卵管炎症大鼠模型 z-bo 2020-12-08 320

　　Objective: To explore the method of constructing a model of chronic salpingitis with stagnation of qi and blood stasis, to investigate its feasibility and to make a preliminary evaluation of the model, so as to lay the foundation for further experimental research.

　　Methods: Select SD rats, use unpredictable repeated chronic stress stimulation (acoustic, light, electrical, tail-clamping stimulation) method and tubal surgery to inject bacteria to construct a model of chronic fallopian tube inflammation with stagnation of qi and blood stasis, and evaluate the symptoms ､Characterization and pathological observation and detection of various indicators of blood rheology, preliminary evaluation of the establishment of an animal model of chronic fallopian tube inflammation with stagnation of qi and blood stasis.

　　Results: Compared with the model group, the total symptom score of the compound model group was significantly increased (P<0.01). In the histomorphology observation, 80% of the fallopian tubes had severe vascular swelling, and 60% had severe tube wall thickening; the pathological morphological analysis found that the composite There were more inflammatory cell infiltrations in the fallopian tube lumen of the model group, mucosal epithelial cells shed, and a large number of inflammatory cell infiltrations around the fallopian tubes; the blood rheology test obtained the plasma viscosity, whole blood viscosity, reduced viscosity, and red blood cell rigidity of the composite model group The index and aggregation index increased significantly (P<0.01).

　　Conclusion: The comprehensive evaluation and analysis found that the chronic salpingitis model rats with qi stagnation and blood stasis basically conformed to the change characteristics of blood stasis syndrome and the pathological manifestations of chronic inflammation, similar to the clinical manifestations of humans in the onset of the disease, and the modeling method is feasible.