认知功能损伤大鼠模型 z-bo 2020-12-08 354

　　Objective: To irradiate the head of rats with different doses, select appropriate doses to establish an animal model of cognitive function impairment caused by ionizing radiation, and provide a research basis for studying the mechanism of cognitive function impairment caused by ionizing radiation and the development of radiation protection agents. Methods: Twenty SPF male rats were randomly divided into control group (group C), 10Gy irradiation group (10GyIR group), 20Gy irradiation group (20GyIR group) and 30Gy irradiation group (30GyIR group), 5 in each group. Group C was left untreated. The remaining 3 groups used 10Gy, 20Gy and 30Gy electronic wires to irradiate the heads of SD rats in different groups with a single shot. The mortality of the animals was calculated 30 days after irradiation. The Morris water maze was used to evaluate the spatial memory of rats. Detection, using chemical colorimetry to detect reduced glutathione (GSH) and malondialdehyde (MDA) in rat brain cortex homogenate, enzyme-linked immunoSorbent assay (ELISA) detection The content of 8-hydroxydeoxyguanosine (8-OHdG) is used to detect and observe the pathological sections of the brain stained by HE. Results: The animals in the 30GyIR group showed signs of irregular back hair, salivation, and severe hair loss in the head and neck. At 30 days after the exposure, no animals died in the C group. The mortality rate of the animals in the 10GyIR group and the 20GyIR group was 20%, and the 30GyIR group The animal mortality rate was 40%. The results of the Morris water maze positioning navigation experiment showed that the incubation period of the 30GyIR group was higher than the other three groups during the 1~5d training period, and the difference was statistically significant (P<0.05). The space search experiment showed that 30GyIR Compared with other groups, the staying time in the original platform quadrant of the group was significantly shorter (P<0.05); the number of crossing platforms was significantly reduced (P<0.05); the swimming distance of the original platform quadrant of the 10GyIR group, the 20GyIR group, and the 30GyIR group was significantly shorter than that of the C group (P <0.05); The test results of oxidative stress-related indicators showed that the GSH, 8-OHdG and MDA concentration differences between the 30Gy IR group and the C group were statistically significant (P<0.05), and the 20Gy IR group and the C group had a statistically significant difference in the MDA concentration Significance (P<0.05); HE stained sections showed that the tissue structure damage in the 20GyIR group and 30GyIR group was more serious than that in the 10GyIR group, and significant pathological changes such as tissue cell apoptosis and necrosis appeared.

　　 Conclusion: The results of this experiment suggest that a single 30Gy irradiation of the head can successfully establish an animal model of cognitive dysfunction in rats.