大鼠恐惧应激模 z-bo 2020-12-08 308

　　Objective: To construct rat models of varying degrees of fear stress and explore the effect of fear stress on the visual cognition of LE rats. Methods: Using plantar electric shock as a stress stimulus, a cognitive choice experiment was designed, and neural response signals from the amygdala brain area were collected for functional network analysis to evaluate the visual cognitive effects of rats. First, the experimental rats were divided into strong (S+). The weak (S) fear stress group and the control group (N) were set up with different intensities of plantar electric shock stimulation respectively; then they were subjected to a single graphic "△" visual cognitive enhancement training; finally, a double picture ("△" "And "10" graphics) to conduct a visual choice test experiment. In addition, combined with complex network theory, the visual cognitive function network of the amygdala nerve nucleus of rats with fear stress is constructed, and the brain function network is characterized by the average path length and clustering coefficient The efficiency of information transmission. Results: The time required to complete the visual cognitive intensive training was significantly higher in the S+ group than in the S､N group. The S group in the early stage of the intensive training was significantly higher than the N group, and there was no significant difference between the two groups in the later stage. In the cognitive choice experiment, the S group was N group formed visual cognitive connection, while S+ group did not form visual cognitive connection; in brain function network analysis, effective visual information transmission was formed between S group and N group of amygdala neurons, while S+ group did not.

　　 Conclusion: Fear stress has a negative impact on visual cognition, and the cognitive effect becomes significantly worse as the degree of fear increases.