白血病模型 z-bo 2020-12-08 374

　　Purpose: To construct an M1 leukemia model using BALB/c nude mice. Methods: Twelve female BALB/cNude mice aged 5-6 weeks were randomly divided into low, medium, and high-dose groups and a blank group, with 3 mice in each group, which were injected via tail vein, low, medium, and high-dose groups. Multiple growth phase M1 cell suspension is 1×106 each, 5×106 each, and 8×106 each. Observe the general condition of the mouse, and collect peripherals at 0, 10, 20, and 30 days after modeling Blood, test blood routine, perform white blood cell classification, sacrifice the samples on the 30th day or when dying, flow cytometry to detect the proportion of CD33CD117 positive cells in peripheral blood and bone marrow, and prepare histopathological sections. Results: The mice in the model group showed malaise, low mobility, arched spine and other conditions on the 10th to 15th day after inoculation. On the 30th day of the experiment, compared with the blank group, the number of peripheral blood leukocytes in the high-dose group increased significantly (P<0.05); the proportion of peripheral blood leukemia cells in each group was significantly higher than that in the blank group (P <0.05); the ratio of CD33+CD117+ in the bone marrow of mice in each group increased, and the high-dose group was the most significant (P<0.05); the high-dose group A small amount of leukemia cell infiltration can be seen in the spleen.

　　 Conclusion: BALB/cNude mice can construct an acute myeloid leukemia model after injecting 8×106 mouse leukemia cells M1 through the tail vein, which is in line with the biological characteristics of acute myeloid leukemia.