小鼠过敏性哮喘模型 z-bo 2020-12-08 397

　　Purpose: The mechanism of traditional Chinese medicine to treat diseases by regulating the flora is gradually discovered, to explore the establishment of an asthma model in which antibiotics cause respiratory tract flora disorders, in order to provide a suitable model for the study of the effects of traditional Chinese medicine on the respiratory tract flora and asthma.

　　Methods: 40 SPF male BALB/c mice were randomly divided into the following 4 groups: normal control group, asthma model group, nasal antibiotic asthma model group, intraperitoneal injection of antibiotics asthma model group. House dust mite , HDM) to establish an asthma model. The antibiotic cefoperazone sulbactam sodium (CFP) was administered by nasal drops or intraperitoneal injection, respectively, and the effect of antibiotics on HDM-induced asthma in mice was observed. The mouse eyes were taken 24 days later. Blood, the asthma indicators of each group of mice: blood eosinophils (eosinophils, EOS) were counted, serum IgE levels were detected, mouse alveolar lavage fluid (BALF) and lung tissue homogenates The levels of TH2 cytokines IL-5 and IL-13, mouse lung tissues were stained with HE, Masson, PAS, and histopathological changes were observed. Results: In the asthma model group, the blood EOS, serum IgE, BALF and lung tissue homogenate levels of IL-5 and IL-13 were significantly higher than those in the blank group (P<0.05). The antibiotic group was more asthmatic than the control group. The model group had a more significant increase (P<0.05). Histopathological observations also found that the inflammatory infiltration, extracellular matrix deposition and mucus secretion of the intranasal antibiotics were more severe, while the intraperitoneal antibiotic group was associated with asthma. The index has no significant change compared with the model group. Conclusion: The intranasal administration of the antibiotic cefoperazone and sulbactam sodium to mice can aggravate HDM-induced asthma in mice, suggesting that antibiotics in the respiratory tract may cause respiratory tract flora disorder and aggravate the occurrence of asthma. This model can be used as a model without feeding GF Under the condition of mice, it is a tool to study Chinese medicine to regulate respiratory tract flora and asthma.