大鼠条件恐惧记忆模型 z-bo 2020-12-08 436

　　Purpose: Use orthogonal experiments to optimize the best experimental parameters for establishing the conditioned fear memory model of rats and find the best experimental conditions.

　　Methods: Using the principle of Paplov’s conditioned fear, a rat conditioned fear memory model was established; a 3-factor 3-level orthogonal experiment design was used to take the rat stupor reaction time 24 hours after the model was established as an indicator to observe fear under different parameter conditions The expression of memory and determine the best test conditions. Establish a conditioned fear memory model based on the optimal experimental conditions, and perform the fear memory retention test after 24h, 1 week, 2 weeks, 4 weeks and 8 weeks.

　　Results: The intuitive analysis results show that the influencing ability of each factor is in the order of sound intensity = number of cycles> electric shock intensity; the results of variance analysis show that the number of cycles has a significant influence on the experimental results (P<0.05), and="" the="" influence="" of="" sound="" intensity="" electric="" shock="" is="" not="" significant="" p="">0.05). The optimal experimental conditions were determined as: sound 75dB, electric shock 0.8mA, and 15 cycles. When the model was established for 24h and 1 week, the rats' fear memory maintained good, and the difference was significant compared with the control group (P< 0.001 and P<0.05); after="" the="" second="" week="" p="">0.05).

　　Conclusion: This experiment clarified the priority of the influencing factors of the rat conditioned fear memory model, optimized the experimental conditions, and provided a reference for the standardization of the model, standardization and follow-up research.