酒精性心肌病小鼠模型 z-bo 2020-12-09 320

　　Objective To establish a mouse model of alcoholic cardiomyopathy with liquid feed and provide a good animal model for the study of the mechanism of alcoholic cardiomyopathy. Methods 16-week-old C57 mice were fed with alcohol-containing liquid feed and non-alcoholic control feed (alcohol group n=21; non-alcohol group n=9), and the alcohol concentration of alcohol-containing feed gradually increased from 4.8% It was as high as 5.4%, fed for 8 weeks, weight measurement was carried out every week, mortality was monitored, and echocardiography and pathological examinations were carried out after 8 weeks. Results The weight of the alcohol-fed group began to decrease from the second week, and it continued until the eighth week that the weight was significantly reduced compared with the control group; at the same time, 7 mice in the alcohol group died during the alcohol-fed period (accounting for 33.3%), and the control group There was no death in the group; the results of cardiac ultrasound showed that the anterior and posterior walls of the ventricle in the alcohol group were significantly thinner than the control group, the ventricular diameter increased, and the cardiac ejection fraction and short axis shortening rate were significantly reduced; the alcohol group was smaller The mouse heart/body mass index was significantly larger than that of the control group, and the HE staining results also confirmed that the heart of the alcohol group mice was significantly enlarged.

　　 Conclusion After 8 weeks of alcoholic liquid feed, the mouse heart showed the typical pathological characteristics of alcoholic cardiomyopathy, such as overall enlargement, thinning of the left ventricular wall, and poor cardiac function. Therefore, this method can successfully prepare a mouse model of alcoholic cardiomyopathy.