学习记忆损伤模型 z-bo 2021-10-27 358

　　Objective To study an effective method to establish a mouse model of learning and memory impairment by avoiding dark and Morris water maze experiments. Methods The mice were intraperitoneally injected with scopolamine 1 mg/kg body weight 20 minutes before the electric shock avoidance training, and 90 mg/kg sodium nitrite was subcutaneously injected immediately after the electric shock training, and the mice were given 35% ethanol 0.1 mL 15 minutes before the next electric shock test /10g, establish a model of memory acquisition, memory consolidation and memory reproduction disorder, measure the dark avoidance latency, error times and probe times of each group of mice 6h, 24h, 30h, 48h after electric shock training, and evaluate the three The sensitivity of each index of the memory impairment model method and the most suitable test time. Mice were intraperitoneally injected with scopolamine 1mg/kg and administrated with 35% ethanol 0.1mL/10g to cause learning and memory impairment. The Morris water maze positioning navigation test and space exploration test were used to test the experimental animals’ sense of spatial position and orientation (space Orientation) to establish the evaluation method of spatial learning and memory impairment mouse model. Results The dark avoidance experiment showed that compared with the blank group, the scopolamine-induced memory impairment model mice significantly shortened the dark avoidance latency at 6h and 24h after the electric shock training, and the number of errors increased significantly (P<0.01, P<0.05); sodium nitrite The memory consolidation disorder model mice were significantly reduced in the dark avoidance latency 30h and 48h after the electric shock training, and the number of errors increased significantly (P<0.01, P<0.05); the memory reproduction disorder model mice caused by ethanol were immediately after the electric shock training 24h Before the test, the mice were dosed with ethanol 15mim. It can be seen that the latency period of the mice was significantly shortened, and the number of errors increased significantly (P<0.05, P<0.01). Compared with the blank group, the number of probes in the memory impairment model mice caused by the three drugs increased at 30h and 48h (P<0.0I). The Morris water maze experiment showed that: compared with the blank group, the scopolamine mouse model group had a significantly longer escape latency for positioning navigation and significantly reduced the number of active exploration and crossing platforms (P<0.01 or P<0.05); there="" was="" no="" significant="" difference="" in="" the="" ethanol="" model="" group="" p="">0.05). Conclusion When using scopolamine and sodium nitrite to construct a mouse model of memory acquisition impairment and memory consolidation in the dark avoidance experiment, the corresponding indicators should be tested at 24h and 30h after modeling; the memory reproduction disorder model caused by ethanol should be tested after electric shock training for 24h. 15 minutes before the second test, the mice were administrated with ethanol and tested. The indicators should be the dark latency, the number of errors and the number of probes. The Morris water maze experiment suggests that scopolamine can impair spatial learning and memory in mice, and can be used as a drug to build a model of spatial learning and memory impairment in mice. Ethanol cannot establish a model of spatial memory impairment in mice.