小鼠局灶性脑缺血模型 z-bo 2020-12-09 537

　　Objective To observe the feasibility of electrocoagulation of the middle cerebral artery in mice by electrocoagulation, causing middle cerebral artery occlusion, and establishing a model of cerebral ischemia in mice. Methods The middle cerebral artery (Middle cerebral artery, MCA) was directly occluded by electrocoagulation to make an adult male Balb/c mouse cerebral ischemia model (model group, n=20); meanwhile, the same batch of Balb/c mice was Mice with the same craniotomy but without electrocoagulation and occlusion of the middle cerebral artery served as the sham operation group (sham operation group, n=20). Modified neurological function severity score (mNSS) was used to evaluate the neurological damage in the model group and sham operation group at 24h and 72h after surgery. Results At 24 hours after operation, a slice of brain tissue from the injured area of Balb/c mice in the model group was taken and stained with hematoxylin and eosin. The microscope showed that the ischemic brain tissue interstitial edema, accompanied by cavity formation, and the local brain tissue structure became It is loose and lightly colored, the number of nerve cells is significantly reduced, the cell body of nerve cells is shrunk, and the outline of some cells is not clear. Different degrees of cell degeneration and necrosis, nuclear pyknosis, and disappearance of nucleoli can be seen. The neurological deficit score of the model group was significantly lower than that of the sham operation group at the 24h postoperatively (P<0.05), and this difference could be maintained until the 72h postoperatively.

　　 Conclusion Electrocoagulation can successfully create a mouse focal cerebral ischemia model in Balb/c mice.