经骨髓注射肺转移和术后复发模型 z-bo 2020-12-10 306

　　Objective To establish a model of lung metastasis and postoperative recurrence via bone marrow injection, reveal the superiority and feasibility of the model constructed by bone marrow injection, and provide a new research basis for studying lung metastasis of malignant tumors.

　　Method The mice were divided into breast pad injection group, subcutaneous injection group, tail vein injection group and bone marrow injection group. Use 4T1 cells to build different models to observe the growth, survival and metastasis efficiency of primary tumors in different groups. Inject different numbers of 4T1 cells to observe the time of lung metastasis. The postoperative recurrence model was constructed by injecting 4T1-luc cells into the bone marrow. The mice were divided into the artificial amputation group, the 3rd day amputation group, the 7th day amputation group and the 10th day amputation group. The lung metastasis of mice was observed by intravital imaging.

　　 Results Compared with breast pad injection and subcutaneous injection, bone marrow injection has no effect on the growth of the primary tumor, but its survival period is significantly shortened. Compared with breast pad injection, subcutaneous injection and tail vein injection, bone marrow injection has the highest lung metastasis efficiency. The bone marrow injection method only needs 1×105 4T1 cells to cause lung metastasis on the 12th day. Model mice injected with bone marrow will still cause lung metastasis and recurrence of primary tumors after amputation. Conclusion The mouse model of lung metastasis via bone marrow injection and the mouse model of postoperative recurrence have been successfully established. The mouse model using this injection method has the characteristics of short survival and high metastasis efficiency, and can be used for mechanism research and drug screening of malignant tumor lung metastasis .