颅骨细胞 z-bo 2020-12-10 297

　　Objective The inbred nude mice (GFP nude mice) of transgenic green fluorescent protein gene established in this study have played an important role in tracing and studying the microenvironment of gliomas, but they have played an important role in the treatment of bone resorption complications after autologous cranioplasty. There is no report on the effect and mechanism. This article aims to analyze the phenotype of GFP nude mouse skull cells cultured in vitro, and lay the foundation for preparing tool cells for treatment of skull resorption. Methods 3 days after birth, GFP nude mice were taken, the bilateral parietal bones were dissected under aseptic conditions, together with the periosteum, the skull was cut into small pieces of about 1 mm2, and placed in 1640 medium containing fetal bovine serum, in 5% CO2 In the incubator for short-term subculture, the cells grown from the bone slices are collected for related testing. Results Observation of primary (P0) and secondary (P1, P2) cells showed that the passage time for 90% of the 60 mm dish bottom was about 6-8 days, and the sampling cell count was about 2.3×106 ~ 2.5× 106 cells/dish, the morphology is mainly fibrous, and there are also stars and dendrites; all cells under a fluorescence microscope all emit green fluorescence, and the morphology is consistent with that under a white light microscope; the detection of marker protein shows that they exist simultaneously in the entire cell population BMP-6+ osteogenic progenitor cells and CD206+ and CD68+ macrophages. Conclusion Based on skull regeneration, in addition to osteoblast progenitor cells as starting cells, macrophages must also be involved in maintaining environmental homeostasis. The successfully cultured P0, P1 and P2 three generations of cells meet this need at the same time, and are expected to be further used as tool cells. Research on skull regeneration.